Selected article for: "monoclonal antibody and polyclonal antibody rabbit"
Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo
  • Document date: 2016_3_14
    • ID: pwlybr2h_23
    Snippet: Immunofluorescence assay (IFA) IFA was performed to detect the hantavirus antigens in the infected Vero-E6 cells as previously reported [18] with monoclonal antibody A35, which targets the nucleocapsid protein (1:200 dilution, provided by the Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China), or rabbit polyclonal antibody targeting glycoprotein G2 (1:100 dilution, provided by Prof John W HUGGINS, Virology Division, Un.....
    Document: Immunofluorescence assay (IFA) IFA was performed to detect the hantavirus antigens in the infected Vero-E6 cells as previously reported [18] with monoclonal antibody A35, which targets the nucleocapsid protein (1:200 dilution, provided by the Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China), or rabbit polyclonal antibody targeting glycoprotein G2 (1:100 dilution, provided by Prof John W HUGGINS, Virology Division, United States Army Medical Research Institute of Infectious Disease). After incubation, FITC-conjugated goat anti-mouse or anti-rabbit IgG (Sigma Aldrich, 1:200 dilution) secondary antibodies were used. The cell cytoplasm was stained red with Evans blue. The images were collected using a fluorescence microscope (Nikon TE2000).

    Search related documents:
    Co phrase search for related documents
    • cell cytoplasm and nucleocapsid protein: 1, 2, 3, 4, 5, 6, 7
    • dilution nucleocapsid protein and nucleocapsid protein: 1
    • FITC conjugate and monoclonal antibody: 1
    • FITC conjugate and polyclonal antibody: 1
    • FITC conjugate and secondary antibody: 1
    • fluorescence microscope and IFA immunofluorescence assay: 1
    • fluorescence microscope and immunofluorescence assay: 1, 2
    • fluorescence microscope and monoclonal antibody: 1, 2, 3, 4, 5, 6
    • fluorescence microscope and nucleocapsid protein: 1
    • fluorescence microscope and polyclonal antibody: 1, 2
    • fluorescence microscope and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • hantavirus antigen and nucleocapsid protein: 1
    • IFA immunofluorescence assay and immunofluorescence assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
    • IFA immunofluorescence assay and monoclonal antibody: 1, 2, 3, 4, 5
    • IFA immunofluorescence assay and nucleocapsid protein: 1, 2, 3, 4, 5, 6, 7, 8
    • IFA immunofluorescence assay and polyclonal antibody: 1, 2, 3, 4
    • IFA immunofluorescence assay and secondary antibody: 1, 2, 3
    • immunofluorescence assay and monoclonal antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • immunofluorescence assay and nucleocapsid protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25