Author: Li, Ji Lian; Cornman, R. Scott; Evans, Jay D.; Pettis, Jeffery S.; Zhao, Yan; Murphy, Charles; Peng, Wen Jun; Wu, Jie; Hamilton, Michele; Boncristiani, Humberto F.; Zhou, Liang; Hammond, John; Chen, Yan Ping
Title: Systemic Spread and Propagation of a Plant-Pathogenic Virus in European Honeybees, Apis mellifera Document date: 2014_1_21
ID: wxiazglk_27
Snippet: Primers specific for TRSV RNA segments 1 and 2 were designed based on the nucleotide sequences obtained from cDNA clones of this study. The sequences of primers for amplifying a 462-bp region of helicase (Hel) of RNA segment 1 were TRSV-F1 (5=-CATGAATGTTGTTATCCAAT-3=) and TRSV-R1 (5=-TCCTCAGTAAATTTCATTTG-3=). The sequences of primers for amplifying a 731-bp region of capsid protein (CP) region of RNA segment 2 were TRSV-F2 (5=-GTGTGCTGTGACGGTTGTT.....
Document: Primers specific for TRSV RNA segments 1 and 2 were designed based on the nucleotide sequences obtained from cDNA clones of this study. The sequences of primers for amplifying a 462-bp region of helicase (Hel) of RNA segment 1 were TRSV-F1 (5=-CATGAATGTTGTTATCCAAT-3=) and TRSV-R1 (5=-TCCTCAGTAAATTTCATTTG-3=). The sequences of primers for amplifying a 731-bp region of capsid protein (CP) region of RNA segment 2 were TRSV-F2 (5=-GTGTGCTGTGACGGTTGTTCC-3') and TRSV-R2 (5=-TGCCAGACCACCCAAGATTCC-3=). Figure 1 illustrates the positions of primers.
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