The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells

Li, Li; Wang, Li; Xiao, Ruijing; Zhu, Guoguo; Li, Yan; Liu, Changxuan; Yang, Ru; Tang, Zhiqing; Li, Jie; Huang, Wei; Chen, Lang; Zheng, Xiaoling; He, Yuling; Tan, Jinquan

The potential mechanism of TMV-RNA-induced autophagy in HeLa cells Based on the above surprising observations, we performed further experiments to support our hypothesis. As mentioned above, the genomic structure of TMV-RNA functions like that of mRNA in that it can be translated into proteins directly. TMV can utilize the host ER as the primary site of envelope glycoprotein biogen-esis, genomic replication and particle assembly. If the translation of TMV-RNA occurred in the ER, the accumulation of TMV protein would inevitably lead to cellular stress responses, such as ERS, which may promote autophagy in HeLa cells. To test this assumption, three approaches were taken.