Author: Hu, Yongwu; Wen, Jie; Tang, Lin; Zhang, Haijun; Zhang, Xiaowei; Li, Yan; Wang, Jing; Han, Yujun; Li, Guoqing; Shi, Jianping; Tian, Xiangjun; Jiang, Feng; Zhao, Xiaoqian; Wang, Jun; Liu, Siqi; Zeng, Changqing; Wang, Jian; Yang, Huanming
Title: The M Protein of SARS-CoV: Basic Structural and Immunological Properties Document date: 2016_11_28
ID: xzlcyn3l_44
Snippet: The sequences were assembled by using Phrap (http://www.phrap.org). ORFs were identified by ORF Finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html). Composition of the nucleotide sequences was analyzed by DNA_GC_Calculator (http://www.genome.iastate.edu/ftp/share/DNAgcCal/) and hypothesized physical and chemical features of the protein and glycosylation sites were examined by using Compute PI/MW (http://us.expasy.org/tools/pi_tool.html) and Net-N.....
Document: The sequences were assembled by using Phrap (http://www.phrap.org). ORFs were identified by ORF Finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html). Composition of the nucleotide sequences was analyzed by DNA_GC_Calculator (http://www.genome.iastate.edu/ftp/share/DNAgcCal/) and hypothesized physical and chemical features of the protein and glycosylation sites were examined by using Compute PI/MW (http://us.expasy.org/tools/pi_tool.html) and Net-NGlyc1.0 (http://www.cbs.dtu.dk/services/NetNGlyc) respectively. Comparative analysis was performed using Blast against the non-redundant protein and non-redundant nucleotide sequences databases (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi). Multiple sequence alignment was performed with ClustalW1.8 (ftp://ftp-igbmc.u-strasbg.fr/pub/ClustalW). TMHMM (http://www.cbs.dtu.dk/services/TMHMM-2.0/), TMMTOP (http://www.enzim.hu/hmmtop/index.html) and Antheprot 5.0 (http://antheprot-pbil.ibcp.fr) were used for transmembrane and secondary structure prediction. Protease cleavage sites were analysed by PeptideCutter (http://ca.expasy.org/tools/peptidecutter/).
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