Selected article for: "allantoic cavity and spf chicken"
Author: Li, Hai; Wang, Fengjie; Han, Zongxi; Gao, Qi; Li, Huixin; Shao, Yuhao; Sun, Nana; Liu, Shengwang
Title: Genome-Wide Gene Expression Analysis Identifies the Proto-oncogene Tyrosine-Protein Kinase Src as a Crucial Virulence Determinant of Infectious Laryngotracheitis Virus in Chicken Cells
  • Document date: 2015_12_17
    • ID: qwrdr92h_7
    Snippet: Experimental animals. The allantoic cavity of 9-day-old specificpathogen-free (SPF) chicken embryos was inoculated with 100 l of virus specimens at 1 Ï« 10 4 or 1 Ï« 10 5 50% tissue culture infective doses (TCID 50 ) per ml, as indicated below, as well as 25 to 250 ng of SU6656 (Selleckchem, Houston, TX, USA), 250 ng of PF573228 (Sigma-Aldrich, St. Louis, MO, USA), or an equal volume of dimethyl sulfoxide (DMSO). All chicken embryos were incubate.....
    Document: Experimental animals. The allantoic cavity of 9-day-old specificpathogen-free (SPF) chicken embryos was inoculated with 100 l of virus specimens at 1 ϫ 10 4 or 1 ϫ 10 5 50% tissue culture infective doses (TCID 50 ) per ml, as indicated below, as well as 25 to 250 ng of SU6656 (Selleckchem, Houston, TX, USA), 250 ng of PF573228 (Sigma-Aldrich, St. Louis, MO, USA), or an equal volume of dimethyl sulfoxide (DMSO). All chicken embryos were incubated at 37°C and examined daily over 5 days. The chorioallantoic membranes (CAMs), allantoic fluids, and livers were then harvested, homogenized, and subjected to three cycles of freezethawing. Hematoxylin and eosin (H&E) staining was performed on sections of liver samples fixed with 4% paraformaldehyde as previously described (36) .

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