Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_11
Snippet: Determination of mitochondrial membrane potential (MMP). At 24 h following transfection, the culture medium of the NCI-H292 cells was replaced with DMEM that did not include phenol red and was supplemented with 5 mg/l JC-1 dye (Beyotime Institute of Biotechnology) in the dark for 20 min at 37ËšC. Subsequently, the cells were washed twice with PBS and placed in fresh medium without serum. Finally, MMP was analyzed by calculating the ratio of fluor.....
Document: Determination of mitochondrial membrane potential (MMP). At 24 h following transfection, the culture medium of the NCI-H292 cells was replaced with DMEM that did not include phenol red and was supplemented with 5 mg/l JC-1 dye (Beyotime Institute of Biotechnology) in the dark for 20 min at 37ËšC. Subsequently, the cells were washed twice with PBS and placed in fresh medium without serum. Finally, MMP was analyzed by calculating the ratio of fluorescence intensity at 555-488 nm in triplicate.
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