Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_18
Snippet: Previous studies have revealed that the NS1 protein of H5N1 is able to induce apoptosis in A549 cells and human airway epithelial cells (17, 18) . The present study attempted to clarify whether the expression of NS1 was sufficient to induce apoptosis with or without STS, which was a confirmed apoptosis inducer, and STS (0.025 nM) was used as a positive control. Initially, NS1 protein and STS were used to induce cytotoxicity, and the cytotoxicity .....
Document: Previous studies have revealed that the NS1 protein of H5N1 is able to induce apoptosis in A549 cells and human airway epithelial cells (17, 18) . The present study attempted to clarify whether the expression of NS1 was sufficient to induce apoptosis with or without STS, which was a confirmed apoptosis inducer, and STS (0.025 nM) was used as a positive control. Initially, NS1 protein and STS were used to induce cytotoxicity, and the cytotoxicity was then detected under various conditions using the MTT assay. Compared with cells transfected with empty vector, the viability of NS1-transfected cells was markedly lower (Fig. 2) . However, the addition of STS significantly decreased the viability of the cells compared with NS1-transfected cells and STS-treated cells, which suggested an increase in cytotoxicity (P<0.05).
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