Author: Zeng, Zhengyang; Zhang, Runhong; Hong, Wei; Cheng, Yuting; Wang, Huijuan; Lang, Yange; Ji, Zhenglin; Wu, Yingliang; Li, Wenxin; Xie, Youli; Cao, Zhijian
Title: Histidine-rich Modification of a Scorpion-derived Peptide Improves Bioavailability and Inhibitory Activity against HSV-1 Document date: 2018_1_1
ID: zilqyfjl_37
Snippet: DNA content was quantified by real-time PCR after total DNA extraction using the MiniBEST Viral RNA/DNA Extraction Kit (Takara, Minamikusatsu Station, JPN) with elution in 50 µL distilled water. Real-time PCR was performed using a SYBR Green PCR assay kit and an ABI7500 system. The forward primer sequence was 5'-CATCACCGACCCGGAGAGGGAC-3', and the reverse primer sequence was 5'-GGGCCAGGCGCTTGTTGGTGTA-3'. PCR products were subjected to a melting c.....
Document: DNA content was quantified by real-time PCR after total DNA extraction using the MiniBEST Viral RNA/DNA Extraction Kit (Takara, Minamikusatsu Station, JPN) with elution in 50 µL distilled water. Real-time PCR was performed using a SYBR Green PCR assay kit and an ABI7500 system. The forward primer sequence was 5'-CATCACCGACCCGGAGAGGGAC-3', and the reverse primer sequence was 5'-GGGCCAGGCGCTTGTTGGTGTA-3'. PCR products were subjected to a melting curve analysis to confirm amplification specificity.
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