Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis Document date: 2010_11_15
ID: ufw13pjx_22
Snippet: The E CT peptide (position 34–76) was designed based on the amino acid sequence of the E envelope protein of the SARS-CoV Urbani strain (accession number: AY278741). The CRB3 CT peptide was prepared as described previously (Makarova et al., 2003). These peptides were purchased from Peptide 2.0 (Chantilly, VA) and were dissolved in 4% dimethyl sulfoxide (DMSO, Sigma Aldrich). The peptides were used at 200 μM and 1 mM in competition assays......
Document: The E CT peptide (position 34–76) was designed based on the amino acid sequence of the E envelope protein of the SARS-CoV Urbani strain (accession number: AY278741). The CRB3 CT peptide was prepared as described previously (Makarova et al., 2003). These peptides were purchased from Peptide 2.0 (Chantilly, VA) and were dissolved in 4% dimethyl sulfoxide (DMSO, Sigma Aldrich). The peptides were used at 200 μM and 1 mM in competition assays.
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