Title: The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope Document date: 1992_12_2
ID: vznqgnzd_10
Snippet: To distinguish the expression of recombinant gp210 from the endogenous protein, an epitope tag encoding a 12-amino acid residue peptide from the influenza virus HA molecule (8, 34) , with two flanking glycine residues added as spacers, was inserted into gp210 after amino acid residue 28. This was accomplished by synthesizing two complimentary oligonucleotides, HA-1 and HA-2, with the following sequence......
Document: To distinguish the expression of recombinant gp210 from the endogenous protein, an epitope tag encoding a 12-amino acid residue peptide from the influenza virus HA molecule (8, 34) , with two flanking glycine residues added as spacers, was inserted into gp210 after amino acid residue 28. This was accomplished by synthesizing two complimentary oligonucleotides, HA-1 and HA-2, with the following sequence.
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