Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_14
Snippet: Leaf samples were homogenised in 250 mM Tris HCl (pH 7.8), mixed with equal volume of Laemmli buffer (2x), boiled for 5 min, clarified by centrifugation and separated on a 12% SDS polyacrylamide gel. Each sample was comprised of three discs obtained from the different agroinfiltrated leaves of one plant. Coomassie staining was performed by Coomassie blue. The scFv-TM43-E10 blots were probed with an anti-c-Myc mouse antibody (Sigma-Aldrich). The s.....
Document: Leaf samples were homogenised in 250 mM Tris HCl (pH 7.8), mixed with equal volume of Laemmli buffer (2x), boiled for 5 min, clarified by centrifugation and separated on a 12% SDS polyacrylamide gel. Each sample was comprised of three discs obtained from the different agroinfiltrated leaves of one plant. Coomassie staining was performed by Coomassie blue. The scFv-TM43-E10 blots were probed with an anti-c-Myc mouse antibody (Sigma-Aldrich). The scFv-Fc-TM43-E10 membranes were developed using an anti-human lgG (Fc) mouse antibody (Sigma-Aldrich). Crossreacting protein bands with secondary anti-mouse goat antibody (Sigma-Aldrich) were visualized with BCIP/NBT color developing reagent.
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