Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_14
Snippet: Leaf samples were homogenised in 250 mM Tris HCl (pH 7.8), mixed with equal volume of Laemmli buffer (2x), boiled for 5 min, clarified by centrifugation and separated on a 12% SDS polyacrylamide gel. Each sample was comprised of three discs obtained from the different agroinfiltrated leaves of one plant. Coomassie staining was performed by Coomassie blue. The scFv-TM43-E10 blots were probed with an anti-c-Myc mouse antibody (Sigma-Aldrich). The s.....
Document: Leaf samples were homogenised in 250 mM Tris HCl (pH 7.8), mixed with equal volume of Laemmli buffer (2x), boiled for 5 min, clarified by centrifugation and separated on a 12% SDS polyacrylamide gel. Each sample was comprised of three discs obtained from the different agroinfiltrated leaves of one plant. Coomassie staining was performed by Coomassie blue. The scFv-TM43-E10 blots were probed with an anti-c-Myc mouse antibody (Sigma-Aldrich). The scFv-Fc-TM43-E10 membranes were developed using an anti-human lgG (Fc) mouse antibody (Sigma-Aldrich). Crossreacting protein bands with secondary anti-mouse goat antibody (Sigma-Aldrich) were visualized with BCIP/NBT color developing reagent.
Search related documents:
Co phrase search for related documents- anti human lgG Fc mouse and mouse antibody: 1
- anti human lgG Fc mouse antibody and goat antibody: 1
- anti human lgG Fc mouse antibody and mouse antibody: 1
- anti mouse goat antibody and equal volume: 1
- anti mouse goat antibody and goat antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
- anti mouse goat antibody and mouse antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
- anti mouse goat antibody and pH Tris HCl: 1
- anti mouse goat antibody and polyacrylamide gel: 1
- anti mouse goat antibody and SDS polyacrylamide gel: 1
- anti mouse goat antibody and Sigma Aldrich anti mouse goat antibody: 1
- anti mouse goat antibody and Tris HCl: 1
Co phrase search for related documents, hyperlinks ordered by date