Transient plant production of Salmonella Typhimurium diagnostic antibodies

Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael

Recombinant proteins in plants can be produced using two main expression methods: stable transformation and transient expression [15] [16] [17] . Amongst the several expression approaches in plants transient expression techniques, especially techniques based on plant virus vectors, made the most significant progress in recent years. Two main approaches, "full-length" strategy and deconstructed strategy, have been used to design virus vectors [18] [19] [20] . Deconstructed versions of RNA viruses like, Potato virus X [21] , Tobacco mosaic virus [22, 23] , Cowpea mosaic virus [24] , as well as DNA viruses like Beet curly top virus [25] , have been developed and successfully applied to produce recombinant proteins in plants. In the most advanced version the "deconstructed virus vectors" are combined with A. tumefaciens-mediated delivery into plant cells [26] .