Selected article for: "digested plasmid and dna fragment"
Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies
  • Document date: 2019_2_12
    • ID: y47ahl3p_7
    Snippet: The pLH-PVX-m vector was constructed by overlap PCR using PVX-AvrI-forw/PVX-ovl-rev and PVX-ovl-forw/PVX-SacI-rev primer pairs (Table S1 ) and a pPVX-201 plasmid [30] as a template. The amplification products were mixed and subjected to a second PCR with PVX-AvrI-forw/PVX-SacI-rev primers. The final PCR fragment was inserted into the pUC-AP [31] vector yielding the pUC-3 0 -PVXm plasmid. The AvrII-SacI fragment of pUC-3 0 -PVX-m was ligated into .....
    Document: The pLH-PVX-m vector was constructed by overlap PCR using PVX-AvrI-forw/PVX-ovl-rev and PVX-ovl-forw/PVX-SacI-rev primer pairs (Table S1 ) and a pPVX-201 plasmid [30] as a template. The amplification products were mixed and subjected to a second PCR with PVX-AvrI-forw/PVX-SacI-rev primers. The final PCR fragment was inserted into the pUC-AP [31] vector yielding the pUC-3 0 -PVXm plasmid. The AvrII-SacI fragment of pUC-3 0 -PVX-m was ligated into pPVX-201 resulting in pPVX-201-m. The modified PVX sequence was transferred into the binary vector pLH-Dbar [32] by ligation of a T4 DNA polymerase treated SphI-EheI fragment of pPVX-201-m and StuI digested pLH-Dbar plasmid. The resulting vector was designated as pLH-PVX-m.

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