Author: Ortega, Ariadna Flores; Martínez-Castañeda, José Simón; Bautista-Gómez, Linda G.; Muñoz, Raúl Fajardo; Hernández, Israel Quijano
Title: Identification of co-infection by rotavirus and parvovirus in dogs with gastroenteritis in Mexico Document date: 2017_6_24
ID: rhsne157_8
Snippet: In the case of parvovirus, 100 ng of DNA from each sample was used for PCR. Previously, we designed a pair of primers: ParvoInt2FB (5 -TCAAGCAGATGGTGATCCAAG-3 ) and ParvoInt2CR The following conditions were used in all PCR reactions: one cycle at 94 • C for 5 min, 35 cycles at 94 • C for 30 s, 52 • C for 1 min, 72 • C for 1 min and a final extension at 72 • C for 5 min. The electrophoresis of amplicons was achieved in a 2% agarose gel (.....
Document: In the case of parvovirus, 100 ng of DNA from each sample was used for PCR. Previously, we designed a pair of primers: ParvoInt2FB (5 -TCAAGCAGATGGTGATCCAAG-3 ) and ParvoInt2CR The following conditions were used in all PCR reactions: one cycle at 94 • C for 5 min, 35 cycles at 94 • C for 30 s, 52 • C for 1 min, 72 • C for 1 min and a final extension at 72 • C for 5 min. The electrophoresis of amplicons was achieved in a 2% agarose gel (stained with 0.5 mg/mL ethidium bromide) and visualized in a UV Bio-Imaging Systems Mini Bis Pro transilluminator (Fig. 1) .
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