Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_43
Snippet: RNAi-mediated protein depletion. For siRNA transfection, 0.4 Ï« 10 5 HEK293 cells were seeded in 6-well plates. Transfections were carried out for 72 h using 20 nM ON-TARGET plus Smart Pool siRNAs (Dharmacon) for one-step depletion (human RNase L and Xrn1) or 100 nM ON-TARGET plus Smart Pool siRNAs (Dharmacon) for two-step depletion (human Drosha, Dicer, and Ago2) with Lipofectamine 2000 (Invitrogen) following the manufacturer's instructions. The.....
Document: RNAi-mediated protein depletion. For siRNA transfection, 0.4 Ï« 10 5 HEK293 cells were seeded in 6-well plates. Transfections were carried out for 72 h using 20 nM ON-TARGET plus Smart Pool siRNAs (Dharmacon) for one-step depletion (human RNase L and Xrn1) or 100 nM ON-TARGET plus Smart Pool siRNAs (Dharmacon) for two-step depletion (human Drosha, Dicer, and Ago2) with Lipofectamine 2000 (Invitrogen) following the manufacturer's instructions. The ON-TARGET plus Nontargeting Pool siRNAs (Dharmacon) was used as a negative control. Cells were infected with SINV and harvested after 16 h for RNA and protein analysis.
Search related documents:
Co phrase search for related documents- Dharmacon negative control and sirna transfection: 1
- human rnase and negative control: 1
- negative control and sirna transfection: 1, 2, 3, 4, 5, 6
- negative control and step depletion: 1
Co phrase search for related documents, hyperlinks ordered by date