Author: Stenglein, Mark D.; Sanders, Chris; Kistler, Amy L.; Ruby, J. Graham; Franco, Jessica Y.; Reavill, Drury R.; Dunker, Freeland; DeRisi, Joseph L.
Title: Identification, Characterization, and In Vitro Culture of Highly Divergent Arenaviruses from Boa Constrictors and Annulated Tree Boas: Candidate Etiological Agents for Snake Inclusion Body Disease Document date: 2012_8_14
ID: vkhg20he_40
Snippet: To generate a continuous boa constrictor cell line (JK), tissues from a boa constrictor that had died of lymphoma were collected at necropsy and stored on ice until processing. Tissue was minced with scalpels, incubated overnight in 0.25% trypsin and 1 mM EDTA in saline A at 4°C, and then plated into culture dishes in cMEM and incubated at 30°C. Culture medium was replaced every 5 days. After 5 weeks, proliferation of adherent cells in a kidney.....
Document: To generate a continuous boa constrictor cell line (JK), tissues from a boa constrictor that had died of lymphoma were collected at necropsy and stored on ice until processing. Tissue was minced with scalpels, incubated overnight in 0.25% trypsin and 1 mM EDTA in saline A at 4°C, and then plated into culture dishes in cMEM and incubated at 30°C. Culture medium was replaced every 5 days. After 5 weeks, proliferation of adherent cells in a kidney-derived culture was evident and these cells were subsequently passaged weekly.
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