Title: In vitro analysis of the oligodendrocyte lineage in mice during demyelination and remyelination Document date: 1990_9_1
ID: vr5hnzp8_19
Snippet: A segment of each spinal cord was excised with a sterile razor blade before glial cell isolation. Segments were fixed by overnight immersion in 1% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer. The tissue was then washed for 1 d in buffer, postfixed with 2 % osmium tetmxide for 2 h, dehydrated in graded alcohols, cleared with propylene oxide, and embedded in Epon resin. Transverse sections (1 ~m) were stained with toluidine blu.....
Document: A segment of each spinal cord was excised with a sterile razor blade before glial cell isolation. Segments were fixed by overnight immersion in 1% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer. The tissue was then washed for 1 d in buffer, postfixed with 2 % osmium tetmxide for 2 h, dehydrated in graded alcohols, cleared with propylene oxide, and embedded in Epon resin. Transverse sections (1 ~m) were stained with toluidine blue.
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