Selected article for: "infectious dose and tissue culture infectious dose"

Author: Falzarano, Darryl; de Wit, Emmie; Martellaro, Cynthia; Callison, Julie; Munster, Vincent J.; Feldmann, Heinz
Title: Inhibition of novel ß coronavirus replication by a combination of interferon-a2b and ribavirin
  • Document date: 2013_4_18
  • ID: tgngoea8_21
    Snippet: Infectivity assay (TCID 50 ). Confluent Vero cells were infected in triplicate with 10fold dilutions of supernatants obtained from the antiviral assay. Virus was allowed to adsorb for 1 h and was then removed and replaced with complete DMEM. Cells were incubated at 37uC, 5% CO 2 in a humidified environment for 5 days and then CPE was scored and TCID 50 (50% tissue culture infectious dose) calculated as described by Reed and Muench 49 ......
    Document: Infectivity assay (TCID 50 ). Confluent Vero cells were infected in triplicate with 10fold dilutions of supernatants obtained from the antiviral assay. Virus was allowed to adsorb for 1 h and was then removed and replaced with complete DMEM. Cells were incubated at 37uC, 5% CO 2 in a humidified environment for 5 days and then CPE was scored and TCID 50 (50% tissue culture infectious dose) calculated as described by Reed and Muench 49 .

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