Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques Document date: 2012_11_30
ID: ted64zo4_11
Snippet: Traditional viral culture requires at least 7 days for yielding a result. To solve this problem, the next step in the viral culture methodology was the introduction of the shellvial assay, based on centrifugation-enhanced adsorption of the sample onto the cells monolayer reproduced on cover slips. Detection is commonly achieved by fluorescent assay with monoclonal antibodies on the cell monolayer. This method reduces the time to obtain a result t.....
Document: Traditional viral culture requires at least 7 days for yielding a result. To solve this problem, the next step in the viral culture methodology was the introduction of the shellvial assay, based on centrifugation-enhanced adsorption of the sample onto the cells monolayer reproduced on cover slips. Detection is commonly achieved by fluorescent assay with monoclonal antibodies on the cell monolayer. This method reduces the time to obtain a result to 24-48 h without a deleterious effect on sensitivity and specificity.
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