Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques Document date: 2012_11_30
ID: ted64zo4_19
Snippet: Most RV are RNA viruses except ADV. Two alternatives are feasible for reversetranscription (RT) of viral RNA into its appropriate cDNA PCR target. One approach is the use of random and/or oligo(dT) primers in the RT step. Subsequently, PCR can be directed against any RV with specific primers. The main disadvantage of this procedure is the need for opening the RT tube to carry out the PCR. Onestep RT-PCR protocols have been reported elsewhere for .....
Document: Most RV are RNA viruses except ADV. Two alternatives are feasible for reversetranscription (RT) of viral RNA into its appropriate cDNA PCR target. One approach is the use of random and/or oligo(dT) primers in the RT step. Subsequently, PCR can be directed against any RV with specific primers. The main disadvantage of this procedure is the need for opening the RT tube to carry out the PCR. Onestep RT-PCR protocols have been reported elsewhere for most RNA viruses, and solve the problem the former protocol has. These are based on the inclusion in the reaction mix of reversetranscriptases and DNA polymerases for both the RT and the PCR steps, or of enzymes with reversetranscriptase and DNA polymerase activities.
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