Laboratory Detection of Respiratory Viruses by Automated Techniques

Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María

Other automated detection formats have been used for RV NAAT, as Respifinder ® 15 assay (PathoFinder) and Seeplex ® RV15 (Seegene). Both detect 15 viral pathogens in respiratory samples. The former is based on a multiplex RT-PCR followed a MLPA reaction, which comprises a probe hybridization step, a probe ligation step and a probe amplification step. Only two primers are used in the final PCR. Every target results in a PCR fragment of a specific length [45] . Seeplex ® RV15 uses dual-priming oligonucleotides that allow multiplex PCR of high specificity and minimizes the possibility of unspecific DNA products. Detection of amplicons is accomplished by capillary electrophoresis with both assays [46] . Although these systems do not avoid the potential of contamination with amplicons, they are able to detect a wide range of RV at a lower cost. However, specific instruments for capillary electrophoresis are available only in high-throughput laboratories.