Selected article for: "ice cold and room temperature"

Author: Dejnirattisai, Wanwisa; Webb, Andrew I.; Chan, Vera; Jumnainsong, Amonrat; Davidson, Andrew; Mongkolsapaya, Juthathip; Screaton, Gavin
Title: Lectin Switching During Dengue Virus Infection
  • Document date: 2011_6_15
  • ID: qos9vu3r_13
    Snippet: Cells were incubated with DENV produced from different cell types at equal amounts of E protein (measured by enzymelinked immunosorbent assay) for 2 h at room temperature. After washing with ice-cold FW, cells were fixed with 4% paraformaldehyde/PBS, and surface-bound virus was detected with an anti-E mAb and anti-mouse IgG/PE followed by FACS analysis......
    Document: Cells were incubated with DENV produced from different cell types at equal amounts of E protein (measured by enzymelinked immunosorbent assay) for 2 h at room temperature. After washing with ice-cold FW, cells were fixed with 4% paraformaldehyde/PBS, and surface-bound virus was detected with an anti-E mAb and anti-mouse IgG/PE followed by FACS analysis.

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