Title: Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence Document date: 1993_3_1
ID: qt44izzh_33
Snippet: were then made, to determine whether additional sequences were also essential for TON localization. Interestingly, every internal deletion mutant displayed a characteristic TGN pattern of localization in transiently transfected NRK cells (Fig. 4 , IDI-ID5), with only a minority of cells having distinct plasma membrane staining. In addition to the TON, some cells showed punctate staining of the cytoplasm (not shown in the figure) , which was mor.....
Document: were then made, to determine whether additional sequences were also essential for TON localization. Interestingly, every internal deletion mutant displayed a characteristic TGN pattern of localization in transiently transfected NRK cells (Fig. 4 , IDI-ID5), with only a minority of cells having distinct plasma membrane staining. In addition to the TON, some cells showed punctate staining of the cytoplasm (not shown in the figure) , which was more pronounced for some of the Fixed, permeabilized cells were stained with a mouse monoclonal antibody to Tac (7G'/) and rhodamine-conjugated antibodies to mouse IgG. Double labeling with antibodies against TGN38 revealed a perfect co-distribution of those chimeras showing TGN-like staining, with TGN38 (data not shown). Bar, 10 #m.
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