Selected article for: "immunofluorescence assay and reduction neutralization"

Author: Jeong, Soo Young; Sung, Se In; Sung, Ji-Hee; Ahn, So Yoon; Kang, Eun-Suk; Chang, Yun Sil; Park, Won Soon; Kim, Jong-Hwa
Title: MERS-CoV Infection in a Pregnant Woman in Korea
  • Document date: 2017_8_8
  • ID: r8molh8c_7
    Snippet: A systematic testing procedure for coronavirus infection, including chest radiograph and serial reverse transcription (RT)-PCR assays with peripheral blood and nasopharyngeal swab, did not demonstrate the presence of MERS-CoV in the newborn. MERS-CoV antibody tests were performed with mother and newborn sera on June 16 and June 28, respectively (5). In the mother's serum, immunoglobulin G (IgG) was detected, albeit weakly, (0.302) via enzyme-link.....
    Document: A systematic testing procedure for coronavirus infection, including chest radiograph and serial reverse transcription (RT)-PCR assays with peripheral blood and nasopharyngeal swab, did not demonstrate the presence of MERS-CoV in the newborn. MERS-CoV antibody tests were performed with mother and newborn sera on June 16 and June 28, respectively (5). In the mother's serum, immunoglobulin G (IgG) was detected, albeit weakly, (0.302) via enzyme-linked immunosorbent assay (ELISA; Euroimmun AG, Luebeck, Germany), and via indirect immunofluorescence test (IIFT; Euroimmun AG) with a titer of 1:100. IgM and IgA were not detected through ELISA and the plaque reduction neutralization test (PRNT) result was below the cutoff value. However, MERS antibodies for IgG, IgM, and IgA were not detected in the newborn's blood samples ( Table 1) .

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