Selected article for: "PI propidium iodide and propidium iodide"
Author: He, Yang; Cao, Lingling; Wang, Li; Liu, Lingping; Huang, Ying; Gong, Xuan
Title: Metformin Inhibits Proliferation of Human Thyroid Cancer TPC-1 Cells by Decreasing LRP2 to Suppress the JNK Pathway
  • Document date: 2020_1_6
    • ID: uzatktbf_9
    Snippet: Cells were washed with phosphate-buffered saline (PBS) twice, centrifuged at 3000 r/min for 5 min and prepared for suspension (5×10 4 /mL). Cell suspension was diluted in 500 μL of binding buffer, incubated with 5 μL of Annexin V in dark for 15 min, and 5 μL of Propidium Iodide (PI) at 4°C, in the dark for another 15 min. After 5-min centrifugation at 3000 r/min, the precipitant was dissolved in 300 μL of binding buffer and subjected to flo.....
    Document: Cells were washed with phosphate-buffered saline (PBS) twice, centrifuged at 3000 r/min for 5 min and prepared for suspension (5×10 4 /mL). Cell suspension was diluted in 500 μL of binding buffer, incubated with 5 μL of Annexin V in dark for 15 min, and 5 μL of Propidium Iodide (PI) at 4°C, in the dark for another 15 min. After 5-min centrifugation at 3000 r/min, the precipitant was dissolved in 300 μL of binding buffer and subjected to flow cytometry.

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