Author: Ma, J; Wan, J; Meng, J; Banerjee, S; Ramakrishnan, S; Roy, S
Title: Methamphetamine induces autophagy as a pro-survival response against apoptotic endothelial cell death through the Kappa opioid receptor Document date: 2014_3_6
ID: wu2mogfa_24
Snippet: Transmission electron microscopy. Transmission Electron Microscopy (TEM) of HUVECs was carried out as previously described 40 on cellular pellets obtained after centrifugation at 1000 Â g for 5 min. Cell pellets were fixed with 2.5% glutaraldehyde for 40 min. After three washes with 0.1 M sodium cacodylate, samples were post-fixed with 1% osmium tetroxide (OsO 4 ) for 30 min. Following three rinses, samples were dehydrated in a graded series of .....
Document: Transmission electron microscopy. Transmission Electron Microscopy (TEM) of HUVECs was carried out as previously described 40 on cellular pellets obtained after centrifugation at 1000 Â g for 5 min. Cell pellets were fixed with 2.5% glutaraldehyde for 40 min. After three washes with 0.1 M sodium cacodylate, samples were post-fixed with 1% osmium tetroxide (OsO 4 ) for 30 min. Following three rinses, samples were dehydrated in a graded series of ethanol. Samples were subsequently embedded in Epon 812 resin. Thin sections (65 nm) were cut by using a Leica UC6 ultramicrotome and stained with uranyl acetate and lead citrate, then examined using a JEOL 1200EX electron microscope (JEOL, Peabody, MA, USA).
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