Author: Ma, J; Wan, J; Meng, J; Banerjee, S; Ramakrishnan, S; Roy, S
Title: Methamphetamine induces autophagy as a pro-survival response against apoptotic endothelial cell death through the Kappa opioid receptor Document date: 2014_3_6
ID: wu2mogfa_28
Snippet: Assessment of apoptosis. Apoptosis was determined by Annexin V staining after METH 72 h treatment. Cells were stained using an Annexin V (green) -FLUOS assay kit (Roche Diagnostics, Indianapolis,IN, USA), and nuclei (blue) with DAPI and analyzed by fluorescence microscopy. Images were recorded with a Nikon confocal microscope at  400 magnification. In some experiments, Annexin V-PE Apoptosis Detection Kit I (BD Biosciences, San Diego, CA, USA) .....
Document: Assessment of apoptosis. Apoptosis was determined by Annexin V staining after METH 72 h treatment. Cells were stained using an Annexin V (green) -FLUOS assay kit (Roche Diagnostics, Indianapolis,IN, USA), and nuclei (blue) with DAPI and analyzed by fluorescence microscopy. Images were recorded with a Nikon confocal microscope at  400 magnification. In some experiments, Annexin V-PE Apoptosis Detection Kit I (BD Biosciences, San Diego, CA, USA) was used to detect apoptosis.
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