Title: Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network Document date: 1991_12_2
ID: syyi2ysq_12
Snippet: (wt/vol) Triton X-100, 1% (wt/vol) sodium deoxycholate, 0.1% (wt/vol) SDS, 0.15 M NaCl, and centrifuged for 5 min at 14,000 rpm in an Eppendorf centrifuge . Aliquots of the supernatant were subjected to SDS-PAGE followed by fluorography. To selectively label the granins present in the TUN, control and hormone-treated cells were preincubated for 30 min with sulfate-free DMEM/Hams F10 and then pulse labeled for 4 min with fresh sulfate-free medium .....
Document: (wt/vol) Triton X-100, 1% (wt/vol) sodium deoxycholate, 0.1% (wt/vol) SDS, 0.15 M NaCl, and centrifuged for 5 min at 14,000 rpm in an Eppendorf centrifuge . Aliquots of the supernatant were subjected to SDS-PAGE followed by fluorography. To selectively label the granins present in the TUN, control and hormone-treated cells were preincubated for 30 min with sulfate-free DMEM/Hams F10 and then pulse labeled for 4 min with fresh sulfate-free medium containing 1 mCi/ml [35 S]sulfate in the absence and presence of the hormone cocktail, respectively.
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