Author: Zhou, Yan; Zheng, HaiHong; Gao, Fei; Tian, DeBin; Yuan, ShiShan
Title: Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase Document date: 2011_9_16
ID: yo9libo0_9
Snippet: Site-directed PCR mutagenesis was carried out using 18 cycles of 95°C for 20 s, 62 or 58°C for 30 s, and 68°C for 11 min using PfuTurbo Hotstart DNA polymerase (Stratagene, La Jolla, CA, USA), added to the reaction mixture after an initial denaturation (95°C, 5 min). After 18 cycles, the products were extended at 72°C for another 10 min......
Document: Site-directed PCR mutagenesis was carried out using 18 cycles of 95°C for 20 s, 62 or 58°C for 30 s, and 68°C for 11 min using PfuTurbo Hotstart DNA polymerase (Stratagene, La Jolla, CA, USA), added to the reaction mixture after an initial denaturation (95°C, 5 min). After 18 cycles, the products were extended at 72°C for another 10 min.
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