n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils

Wu, Beibei; Wang, Liyin; Jiang, Lili; Dong, Lili; Xu, Fengli; Lu, Yili; Jin, Jiahui; Wang, Zhanyue; Liang, Guang; Shan, Xiaoou

n-butanol extract from Folium isatidis prevents the LPS-induced downregulation of CXCR1, CXCR2 and CD62L. CXCR1, CXCR2 and CD62L may be important in sepsis, however, whether n-butanol extract affects these chemokine receptors remains to be elucidated. Therefore, the present study assessed the effect of the extract on the expression of CXCR1, CXCR2 and CD62L. The isolated neutrophils were pre-incubated with either vehicle or increasing concentrations of extract (100, 250 and 500 µg/ml) for 2 h, and were subsequently treated with LPS (0.5 µg/ml) for 4 h. Subsequent analysis of the cells using flow cytometry indicated that the extract inhibited the LPS-induced downregulation of CXCR1, CXCR2 and CD62L ( Fig. 2A-C) in a dose-dependent manner.