Author: Ding, Peiyang; Zhang, Teng; Li, Yafei; Teng, Man; Sun, Yaning; Liu, Xiao; Chai, Shujun; Zhou, Enmin; Jin, Qianyue; Zhang, Gaiping
Title: Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2 Document date: 2017_7_24
ID: upb97on4_3
Snippet: Nanoparticles orientationally display neutralizing epitopes Medium (DMEM), RPMI-1640, penicillin, and streptomycin were purchased from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS), were purchased from GIBCO (Carlsbad, CA, USA). Carbopol ® 971P NF polymer was purchased from Lubrizol (Cleveland, OH, USA). Freund's adjuvant was purchased from Sigma-Aldrich. The oligopeptide 169-STIDYFQPNNKR was synthesized by GL Biochem (Shanghai, People's Re.....
Document: Nanoparticles orientationally display neutralizing epitopes Medium (DMEM), RPMI-1640, penicillin, and streptomycin were purchased from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS), were purchased from GIBCO (Carlsbad, CA, USA). Carbopol ® 971P NF polymer was purchased from Lubrizol (Cleveland, OH, USA). Freund's adjuvant was purchased from Sigma-Aldrich. The oligopeptide 169-STIDYFQPNNKR was synthesized by GL Biochem (Shanghai, People's Republic of China), a cysteine was added to the N-terminal end of the oligopeptide, and then conjugated to bovine serum albumin (BSA) using 4-(N-maleimidomethyl) cyclohexanecarboxylic acid N-hydroxysuccinimide ester according to the manufacturer's instruction (Sigma-Aldrich).
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