Author: Kim, Ji-Hyeon; Lee, Kyunghyun; Sohn, Woon-Mok; Kim, Ha-Young; Lee, Yu-Ran; Choi, Eun-Jin; So, ByungJae; Jung, Ji-Youl
Title: Necrotizing Enteritis Caused by Pharyngostomum cordatum Infection in a Stray Cat Document date: 2019_2_26
ID: s3agg94u_7
Snippet: Genomic DNA was extracted from paraffin-embedded blocks of small intestine using a QIAamp DNA FFPF Tissue kit (Qiagen, Hilden, Germany), following manufacturer protocol. Partial and complete sequences of 5.8 Sribosomal RNA (rRNA) gene, internal transcribed spacer 2 (ITS2), 28S rRNA gene (GenBank accession number: LC025648.1) were PCR amplified using the following primers: 5'-TGTCGATGAAGAG TG-CAGCCA-3' (forward) and 5'-ATCAGTTACATTGCCACATGC-3' (re.....
Document: Genomic DNA was extracted from paraffin-embedded blocks of small intestine using a QIAamp DNA FFPF Tissue kit (Qiagen, Hilden, Germany), following manufacturer protocol. Partial and complete sequences of 5.8 Sribosomal RNA (rRNA) gene, internal transcribed spacer 2 (ITS2), 28S rRNA gene (GenBank accession number: LC025648.1) were PCR amplified using the following primers: 5'-TGTCGATGAAGAG TG-CAGCCA-3' (forward) and 5'-ATCAGTTACATTGCCACATGC-3' (reverse). Thermocycling conditions were as follows: 30 cycles of 1 min at 94ËšC, 1 min at 55ËšC, and 1 min at 72ËšC. The PCR product was purified using a QIA amp Purification kit (Qiagen) and commercially sequenced (Macrogen, Seoul, Korea).
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