Author: Chebib, Hassan El; Bonville, Cynthia; Domachowske, Joseph B.; Suryadevara, Manika
Title: Neutrophil Activation in Infants Hospitalized With Severe Respiratory Viral Infection Document date: 2016_10_25
ID: rv0ojrjr_2
Snippet: Methods. Hospitalized infants < 2 years of age with a documented vRTI attributed to a single virus and healthy infant controls were included in the study. After obtaining informed consent, whole blood was collected into a syringe coated with EDTA. Neutrophil CD11b expression was determined using flow cytometry, gating on CD16+ granulocytes following exposure to increasing concentrations of CXCL-1 (0–100 nM), a potent inducer of CD11b surface ex.....
Document: Methods. Hospitalized infants < 2 years of age with a documented vRTI attributed to a single virus and healthy infant controls were included in the study. After obtaining informed consent, whole blood was collected into a syringe coated with EDTA. Neutrophil CD11b expression was determined using flow cytometry, gating on CD16+ granulocytes following exposure to increasing concentrations of CXCL-1 (0–100 nM), a potent inducer of CD11b surface expression. A sample of infants with vRTI was seen in follow-up at 2, 6 and 10 weeks post-discharge. Whole blood was obtained at each visit for neutrophil CD11b expression analysis.
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