Selected article for: "complementary dna and RNA virus"
Author: Choi, Kang-Seuk
Title: Newcastle disease virus vectored vaccines as bivalent or antigen delivery vaccines
  • Document date: 2017_7_26
    • ID: vk59ghjm_6
    Snippet: Because NDV is a negative-sense single-stranded RNA virus, a functional viral RNP complex is required for viral transcription and replication. Thus, the reverse genetics technology used to generate recombinant NDV (rNDV) requires a fulllength complementary DNA (cDNA) clone and three helper plasmids encoding the NDV N, P, and L proteins under the transcriptional control of a T7 RNA polymerase promoter [8, 12] . Once simultaneously transfected into.....
    Document: Because NDV is a negative-sense single-stranded RNA virus, a functional viral RNP complex is required for viral transcription and replication. Thus, the reverse genetics technology used to generate recombinant NDV (rNDV) requires a fulllength complementary DNA (cDNA) clone and three helper plasmids encoding the NDV N, P, and L proteins under the transcriptional control of a T7 RNA polymerase promoter [8, 12] . Once simultaneously transfected into permissive cells, the three helper plasmids express the N, P, and L proteins, which then bind to genomic or anti-genomic RNA to form the RNP complex.

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