Author: Menachery, Vineet D.; Eisfeld, Amie J.; Schäfer, Alexandra; Josset, Laurence; Sims, Amy C.; Proll, Sean; Fan, Shufang; Li, Chengjun; Neumann, Gabriele; Tilton, Susan C.; Chang, Jean; Gralinski, Lisa E.; Long, Casey; Green, Richard; Williams, Christopher M.; Weiss, Jeffrey; Matzke, Melissa M.; Webb-Robertson, Bobbie-Jo; Schepmoes, Athena A.; Shukla, Anil K.; Metz, Thomas O.; Smith, Richard D.; Waters, Katrina M.; Katze, Michael G.; Kawaoka, Yoshihiro; Baric, Ralph S.
Title: Pathogenic Influenza Viruses and Coronaviruses Utilize Similar and Contrasting Approaches To Control Interferon-Stimulated Gene Responses Document date: 2014_5_20
ID: s3zeppze_32
Snippet: ChIP-PCR. ChIP analysis was performed using the EpiTect ChIP OneDay kit (Qiagen). Briefly, infected Calu3 cells were cross-linked, harvested, and frozen at Ϫ80°C. Cells were then lysed and chromatin sheared via sonication to generate chromatin fragments between 250 and 1,000 bp. Sonicated samples were then immunoprecipitated with anti-STAT1 (clone C-24; Santa Cruz Biotechnology), anti-H3K4me3 (Qiagen), anti-H3K27me3 (Qiagen), or anti-mouse IgG .....
Document: ChIP-PCR. ChIP analysis was performed using the EpiTect ChIP OneDay kit (Qiagen). Briefly, infected Calu3 cells were cross-linked, harvested, and frozen at Ϫ80°C. Cells were then lysed and chromatin sheared via sonication to generate chromatin fragments between 250 and 1,000 bp. Sonicated samples were then immunoprecipitated with anti-STAT1 (clone C-24; Santa Cruz Biotechnology), anti-H3K4me3 (Qiagen), anti-H3K27me3 (Qiagen), or anti-mouse IgG (Qiagen) as a control.
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