Author: Cao, Rui-Yuan; Xu, Yong-fen; Zhang, Tian-Hong; Yang, Jing-Jing; Yuan, Ye; Hao, Pei; Shi, Yi; Zhong, Jin; Zhong, Wu
Title: Pediatric Drug Nitazoxanide: A Potential Choice for Control of Zika Document date: 2017_2_3
ID: sbf9qi6g_11
Snippet: The infected cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 1 hour at room temperature and then treated with 0.3% Triton X-100 in 10% fetal calf serum for 30 minutes. The cells were then incubated at 37°C for 2 hours with the monoclonal antibody against flavivirus envelope protein (clone D1-4G2-4-15; mouse; 1:1000; Millipore, Billerica, MA) followed by the incubation with Alexa Fluor 488-conjugated secondary ant.....
Document: The infected cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 1 hour at room temperature and then treated with 0.3% Triton X-100 in 10% fetal calf serum for 30 minutes. The cells were then incubated at 37°C for 2 hours with the monoclonal antibody against flavivirus envelope protein (clone D1-4G2-4-15; mouse; 1:1000; Millipore, Billerica, MA) followed by the incubation with Alexa Fluor 488-conjugated secondary antibody. Hoechst33258 dye was used to stain the nucleus. The stained cells were analyzed by fluorescence microscopy.
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