Selected article for: "PCR test and primer pair"
Author: Chen, Wenbing; Li, Shousong; Shao, Biying; Zheng, Teng; Jiang, Shuxun; Huang, Xiaorong; Cai, Kaizhen; Zhang, Zhideng
Title: Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
  • Document date: 2016_11_28
    • ID: r2upa4o0_12
    Snippet: So far as reported, SARS-CoV specific target cDNA fragments have been amplified by single PCR. For identifying SARS-CoV, it is necessary to analyze two or more target sequences. The multiplex PCR can amplify two or more target fragments in one reaction tube simultaneously, thus can effectively reduce the probability of false positive phenomena due to reaction competition between each primer pair. The results in the present study showed that it is.....
    Document: So far as reported, SARS-CoV specific target cDNA fragments have been amplified by single PCR. For identifying SARS-CoV, it is necessary to analyze two or more target sequences. The multiplex PCR can amplify two or more target fragments in one reaction tube simultaneously, thus can effectively reduce the probability of false positive phenomena due to reaction competition between each primer pair. The results in the present study showed that it is feasible to test the SARS-CoV with multiplex PCR because its sensitivity is the same as that of single PCR, and it would be more accurate, time-saving, and costeffective.

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