Selected article for: "culture medium and medium culture"
Author: Sneha Rath; Eliza Prangley; Jesse Donovan; Kaitlin Demarest; Yigal Meir; Ned Wingreen; Alexei Korennykh
Title: Concerted 2-5A-Mediated mRNA Decay and Transcription Reprogram Protein Synthesis in dsRNA Response
  • Document date: 2018_12_4
    • ID: ng5c7xai_46
    Snippet: Lysates were boiled at 95 °C for 10 minutes, then separated on 10% BisTris PAGE gels (Invitrogen). Gels were stained with coomassie for total protein visualization, then analyzed by phosphorimaging (Typhoon FLA 7000, GE). For ribopuromycilation assay, cells were treated with 10 g/ml puromycin (Invitrogen) in culture medium for 5 minutes at 37 °C. Cells were lysed and separated by PAGE as above. For western blotting proteins were transferred .....
    Document: Lysates were boiled at 95 °C for 10 minutes, then separated on 10% BisTris PAGE gels (Invitrogen). Gels were stained with coomassie for total protein visualization, then analyzed by phosphorimaging (Typhoon FLA 7000, GE). For ribopuromycilation assay, cells were treated with 10 g/ml puromycin (Invitrogen) in culture medium for 5 minutes at 37 °C. Cells were lysed and separated by PAGE as above. For western blotting proteins were transferred to PVDF membranes (Life Technologies) and stained with Ponceau Red to visualize total proteins. The membrane was washed and blocked in 5% non-fat dry milk in TBST. Membranes were probed with 1:1000 dilution of mouse anti-

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