Recurrent rhinovirus infections in a child with inherited MDA5 deficiency

Lamborn, Ian T.; Jing, Huie; Zhang, Yu; Drutman, Scott B.; Abbott, Jordan K.; Munir, Shirin; Bade, Sangeeta; Murdock, Heardley M.; Santos, Celia P.; Brock, Linda G.; Masutani, Evan; Fordjour, Emmanuel Y.; McElwee, Joshua J.; Hughes, Jason D.; Nichols, Dave P.; Belkadi, Aziz; Oler, Andrew J.; Happel, Corinne S.; Matthews, Helen F.; Abel, Laurent; Collins, Peter L.; Subbarao, Kanta; Gelfand, Erwin W.; Ciancanelli, Michael J.; Casanova, Jean-Laurent; Su, Helen C.

The human embryonic kidney 293T cell line (ATCC) was cultured in DMEM medium (Gibco) supplemented with 10% FBS (Gibco or Hyclone), 2 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin (all from Gibco), and 55 µM 2-ME (Sigma-Aldrich) for all experiments, or Iscove's Modified Dulbecco's Medium (IMDM; Gibco) supplemented with 10% FBS (Gibco or Hyclone) and 55 µM 2-ME (Sigma-Aldrich) for lentivirus production. The monkey kidney cell line Vero E6 (ATCC) was cultured in DMEM supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, and 55 µM 2-ME. The lung epithelial carcinoma cell line A549 (ATCC) was cultured in F-12K medium (Gibco) supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, and 55 µM 2-ME. The H1-HeLa cell line (gift from W.-M. Lee, Biological Mimetics, Inc., Frederick, MD) was cultured in MEM suspension medium with Earle's salts and no calcium (Gibco), supplemented with 10% FBS (Hyclone), 1x MEM nonessential amino acids, 2 mM l-glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, 0.1% Pluronic F-68 (all from Gibco), and 55 µM 2-ME (Sigma-Aldrich). H1-Hela cells were maintained in suspension by growth at 37°C on an incubator shaker set at 230 rpm. The Madin-Darby Canine Kidney (MDCK; ATCC) cell line was cultured in DMEM medium (Gibco) supplemented with 10% FBS (Gibco).