Author: Tang, Hei-Man Vincent; Gao, Wei-Wei; Chan, Chi-Ping; Cheng, Yun; Chaudhary, Vidyanath; Deng, Jian-Jun; Yuen, Kit-San; Wong, Chun-Ming; Ng, Irene Oi-Lin; Kok, Kin-Hang; Zhou, Jie; Jin, Dong-Yan
Title: Requirement of CRTC1 coactivator for hepatitis B virus transcription Document date: 2014_11_10
ID: qtoygz6w_28
Snippet: Activation of HBV transcription might accelerate other steps in viral replication. To verify this, we measured the levels of HBV pgRNA and cccDNA in pHBV1.3D-transfected HepG2 cells. Upon pHBV1.3D transfection, basal expression of pgRNA as well as nuclear and cell-free cccDNA was detected ( Figure 3A and B, group 2, and Figure 3C , lane 3). Intriguingly, their expression was boosted when CRTC1/2/3 was overexpressed ( Figure 3A and B, groups 3-5 c.....
Document: Activation of HBV transcription might accelerate other steps in viral replication. To verify this, we measured the levels of HBV pgRNA and cccDNA in pHBV1.3D-transfected HepG2 cells. Upon pHBV1.3D transfection, basal expression of pgRNA as well as nuclear and cell-free cccDNA was detected ( Figure 3A and B, group 2, and Figure 3C , lane 3). Intriguingly, their expression was boosted when CRTC1/2/3 was overexpressed ( Figure 3A and B, groups 3-5 compared to 2 and Figure 3C , lanes 4-6 compared to 3). In other words, overexpression of CRTCs not only stimulates HBV transcription, but also promotes its replication.
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