Research Communications of the 24th ECVIM-CA Congress

Genomic DNA was extracted from three isolates of each of CU and CH recovered from the faeces of healthy dogs and cats. Sequencing was performed using an Illumina MiSeq to generate 250 base paired reads. Reads were trimmed for both length and quality. Contigs were assembled using the Velvet assembler. The concatenated contigs generated for each assembly were quality ranked (by number, size, maximum length and N50) and the three top ranked assemblies were annotated using the Prokka annotation tool. Ribosomal MLST nucleotide sequences were used as a proxy for the core genome to compare the phylogeny of CU and CH with other species in the Campylobacter genus and visualised as a NeighborNet using SplitsTree. Annotated draft genomes were clustered using OrthoMCL and pathogenic traits were investigated in silico using PathogenFinder and Viru-lentPred software.