Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017 Document date: 2017_11_7
ID: roslkxeq_360
Snippet: Fecal DNA extracts (1:10 and 1:20 dilutions (n = 99), 1:50 dilution (n = 1)) from shelter cats were screened for the presence of CPV and FPV using a PCR assay amplifying a 529 bp region of the VP2 gene using primers 555-F and 555-R. PBS processed in parallel with samples from extraction to PCR served as a negative control and DNA from a previously sequenced canine CPV isolate as a positive control. In samples testing positive, the complete VP2 ge.....
Document: Fecal DNA extracts (1:10 and 1:20 dilutions (n = 99), 1:50 dilution (n = 1)) from shelter cats were screened for the presence of CPV and FPV using a PCR assay amplifying a 529 bp region of the VP2 gene using primers 555-F and 555-R. PBS processed in parallel with samples from extraction to PCR served as a negative control and DNA from a previously sequenced canine CPV isolate as a positive control. In samples testing positive, the complete VP2 gene was sequenced to differentiate FPVs from CPVs. CPV DNA was not detected in the stool of any cat. FPV DNA was detected in the stool of 4 cats.
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