RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS

Nine cats with HCM (5.4±4.1 years, 77.8% males; 4Persian, 3Domestic shorthair, 1Sphynx and 1Chartreux) and 6 controls (3.8±1.8 years, 33,3% males, 2Persian, 1Domestic shorthair, 2Maine Coon and 1Norwegian Forest) were included. HCM was defined as LVH ≥6mm, when other causes of LVH were excluded. DNA was extracted from peripheral blood sample (left‐over from routine haematology sample). A customized panel of 18 genes associated with HCM (ACTC1, DES, FLNC, GLA, LAMP2, MYBPC3, MYH7, MYH6, MYL2, MYL3, PLN, PRKAG2, PTPN11, TNNC1, TNNI3, TNNT2, TPM1, TTR) was sequenced by next generation sequencing. The variants were classified as pathogenic when they were no present in control population and were in conserved domains.