Selected article for: "present study and RT PCR hybridization"

Author: Silva, E.R.M.; Watanabe, A.S.A.; Carraro, E.; Perosa, A.H.S.; Granato, C.F.H.; Bellei, N.C.J.
Title: Rhinovirus detection using different PCR-based strategies
  • Document date: 2012_6_1
  • ID: sluqq1px_14
    Snippet: In conclusion, RT-PCR/hybridization was more sensitive but less specific for rhinovirus detection. The duplex seminested-RT-PCR protocol assessed in the present study was less sensitive and not useful in differentiating HRV from HEV. A sequencing assay using a different gene or genomic approach could determine which protocol is the best strategy to confirm rhinovirus and enterovirus infections......
    Document: In conclusion, RT-PCR/hybridization was more sensitive but less specific for rhinovirus detection. The duplex seminested-RT-PCR protocol assessed in the present study was less sensitive and not useful in differentiating HRV from HEV. A sequencing assay using a different gene or genomic approach could determine which protocol is the best strategy to confirm rhinovirus and enterovirus infections.

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