Author: Ishimaru, Daniella; Plant, Ewan P.; Sims, Amy C.; Yount, Boyd L.; Roth, Braden M.; Eldho, Nadukkudy V.; Pérez-Alvarado, Gabriela C.; Armbruster, David W.; Baric, Ralph S.; Dinman, Jonathan D.; Taylor, Deborah R.; Hennig, Mirko
Title: RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus Document date: 2012_12_26
ID: zrbn637z_12
Snippet: Unlabeled (150 mM) and 32 P-labeled (1 pM) SARS-CoV pk and S3L2 RNA transcripts were annealed in NMR buffer unless stated otherwise. When 5 mM MgCl 2 was added, samples were incubated for 6 h at 37 C. Temperature, time of incubation and RNA concentration varied, as specified in the text. RNA samples were separated on 10% native polyacrylamide gel in Tris Borate buffer pH 8.3 at 4 C when MgCl 2 was added to the dimerization reaction. Otherwise, Tr.....
Document: Unlabeled (150 mM) and 32 P-labeled (1 pM) SARS-CoV pk and S3L2 RNA transcripts were annealed in NMR buffer unless stated otherwise. When 5 mM MgCl 2 was added, samples were incubated for 6 h at 37 C. Temperature, time of incubation and RNA concentration varied, as specified in the text. RNA samples were separated on 10% native polyacrylamide gel in Tris Borate buffer pH 8.3 at 4 C when MgCl 2 was added to the dimerization reaction. Otherwise, Tris Borate EDTA pH 8.3 was used and the gel analysis performed at 4 C or 25 C, as specified in the text. Gels were dried and analysed by phosphorimaging. In Figures 3A and 3B , gels were analysed by ethidium bromide staining.
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