Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Document date: 2014_4_23
ID: xf4yy6i1_31
Snippet: Previous studies have indicated that different reference genes were required for gene expression studies in PBMC at different times or with different immunologic stimulants. For instance, RPL4 and TBP ranked as the two most stably expressed genes in PBMC of pigs at day 1, day 7, day 14 after heat stress, while B2M was the most stable at day 21 after heat stress (Xiang-Hong et al., 2011) ; PPIA, B2M, and RPL4 were the most stable reference genes i.....
Document: Previous studies have indicated that different reference genes were required for gene expression studies in PBMC at different times or with different immunologic stimulants. For instance, RPL4 and TBP ranked as the two most stably expressed genes in PBMC of pigs at day 1, day 7, day 14 after heat stress, while B2M was the most stable at day 21 after heat stress (Xiang-Hong et al., 2011) ; PPIA, B2M, and RPL4 were the most stable reference genes in Lipopolysaccharide (LPS) stimulated PBMC, while Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ), RPL4 and PPIA were the most stably expressed reference genes in the case of lipoteichoicacid (LTA) stimulated PBMC Cinar et al., 2013) . So far, no studies were performed to validate the stability of reference genes in cultured porcine whole blood and PBMC in response to poly I:C stimulation.
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