Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Document date: 2014_4_23
ID: xf4yy6i1_9
Snippet: The other 5 mL fresh blood was diluted by adding 20 mL RPMI-1640 medium supplemented with 10% fetal calf serum, streptomycin and penicillin. About 20 mL diluted whole blood was equally assigned into 16 wells (1.25 mL/well) and the other 5 mL were used as the uncultured and un-stimulated control. According to the sample experimental treatments as PBMC, the diluted whole blood were stimulated and collected, and samples of the three pigs were merged.....
Document: The other 5 mL fresh blood was diluted by adding 20 mL RPMI-1640 medium supplemented with 10% fetal calf serum, streptomycin and penicillin. About 20 mL diluted whole blood was equally assigned into 16 wells (1.25 mL/well) and the other 5 mL were used as the uncultured and un-stimulated control. According to the sample experimental treatments as PBMC, the diluted whole blood were stimulated and collected, and samples of the three pigs were merged.
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