Selected article for: "RNA extraction and RT PCR RNA extraction"
Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage
  • Document date: 2016_4_9
    • ID: z7f720dj_37
    Snippet: BAL samples in which high frequency variants were detected by sequencing were further analyzed using plaque assays and Sanger sequencing. For each sample plaqued, ten plaques were picked for Sanger sequencing of ten genome sites containing the high-frequency mutations. Following RNA extraction, variable regions derived from each plaque were amplified by RT-PCR using PCR primers sets spanning the mutation sites......
    Document: BAL samples in which high frequency variants were detected by sequencing were further analyzed using plaque assays and Sanger sequencing. For each sample plaqued, ten plaques were picked for Sanger sequencing of ten genome sites containing the high-frequency mutations. Following RNA extraction, variable regions derived from each plaque were amplified by RT-PCR using PCR primers sets spanning the mutation sites.

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