Author: Angelini, Megan M.; Akhlaghpour, Marzieh; Neuman, Benjamin W.; Buchmeier, Michael J.
Title: Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles Document date: 2013_8_13
ID: yl1qyh7j_18
Snippet: The precise mechanism by which each of these nsps works to produce double-membrane vesicles is a topic for future study and is likely influenced by a variety of factors, including each nsp's production from the initial polyprotein precursor, how these nsps recruit and exploit host cell proteins, and the interaction of each nsp with other viral proteins and host cell proteins. Antibodies. The primary antibodies used were rabbit anti-nsp3 (Rockland.....
Document: The precise mechanism by which each of these nsps works to produce double-membrane vesicles is a topic for future study and is likely influenced by a variety of factors, including each nsp's production from the initial polyprotein precursor, how these nsps recruit and exploit host cell proteins, and the interaction of each nsp with other viral proteins and host cell proteins. Antibodies. The primary antibodies used were rabbit anti-nsp3 (Rockland) and mouse anti-FLAG (Sigma). Alexafluor-488-and Alexafluor-594-conjugated secondary antibodies (Invitrogen) were used for immunofluorescence. Horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson Laboratories) were used for Western blotting.
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